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Using the same CIDR up to three times for estrus synchronization and artificial insemination in dairy goats = Uso do mesmo CIDR por até três vezes para sincroniza o do estro e insemina o artificial de cabras leiteiras
Daniel Maia Nogueira,Edilson Soares Lopes Júnior,Rodolfo Moraes de Peixoto,Madriano Christilis
Acta Scientiarum : Animal Sciences , 2011,
Abstract: The objective of this study was to evaluate the effectiveness of reusing a controlled internal drug release (CIDR) device for up to three times in the reproductive performance of dairy goats raised in the semi-arid zone of northeastern Brazil. Forty-five goats were allocated into three hormone treatments, as follows: CIDR1x, treated with new CIDR during nine days. Two days prior to device removal, injections of 75 Yg d-cloprostenol and 300 IU equine chorionic gonadotropin (eCG) were administrated. For the other treatments, thesame hormone protocol was used, differing only by the use of the same CIDR for a second time in CIDR2x and for a third time in CIDR3x. The interval from device removal to the onset of estrus (13.3 b 1.1h vs. 13.8 b 2.6h vs. 13.3 b 1.4h), as well as estrus duration (33.6 b 7.3h vs. 29.6 b 3.2h vs. 32.8 b 4.5h), did not differ (p > 0.05) among groups CIDR1x, CIDR2x and CIDR3x, respectively. All synchronized females were found to be in estrus. The overall fertility and prolificacy after artificial insemination were 82.2% and 1.9 kids, respectively, without significant difference (p > 0.05) among treatments. The use of the same CIDR for up to three times waseffective using 9-day estrus synchronization protocols in dairy goats. Objetivou-se avaliar o efeito da utilizacao do mesmo dispositivo de liberacao controlada de drogas (CIDR) por ate tres vezes sobre odesempenho reprodutivo de cabras leiterias exploradas no semiarido do Nordeste Brasileiro. Foram utilizadas 45 cabras divididas em tres tratamentos de sincronizacao do estro, sendo: CIDR1x, tratadas com CIDR novo durante nove dias. Dois dias antes da retirada do dispositivo, foram aplicados 75 Yg de d-cloprostenol e 300 UI de gonadotrofina corionica equina (eCG). Para os demais tratamentos, foi utilizado o mesmo protocolo hormonal, diferindo apenas pelo uso do mesmo CIDR pela segunda vez no grupo CIDR2x e uso pela terceira vez no grupo CIDR3x. O intervalo entre a retirada do dispositivo e o inicio do estro (13,3 b 1,1h vs. 13,8 b 2,6h vs. 13,3 b 1,4h), bem como, a duracao do estro (33,6 b 7,3h vs. 29,6 b 3,2h vs. 32,8 b 4,5h) nao diferiram (p > 0,05) entre os grupos CIDR1x, CIDR2x e CIDR3x, respectivamente. Todas as femeas sincronizadas foram identificadas em estro. As medias de fertilidade e prolificidade media apos inseminacao artificial foram, respectivamente, de 82,2% e 1,9 crias, nao havendo diferenca significativa (p > 0,05) entre os tratamentos. A utilizacao do mesmo CIDR por ate tres vezes foi viavel na sincronizacao do estro de caprinos leiteiros.
ANTIBACTERIAL EFFECT OF BRAZILIAN BROWN PROPOLIS IN DIFFERENT SOLVENTS AGAINST Staphylococcus spp. ISOLATED FROM CAPRINE MASTITIS
Carla Samantha Rodrigues Silva,Claudia Luiza Paes Barreto Villa？a,Rodolfo de Moraes Peixoto,Rinaldo Aparecido Mota
Ciência Animal Brasileira , 2012,
Abstract: The purpose of the present study was to evaluate the antimicrobial activity of propolis extracts diluted in different solvents against bacteria from Staphylococcus genus. The study was performed in the Immunology and Microbiology Laboratory from Universidade Federal do Vale do S o Francisco. The propolis extracts were prepared using brown propolis diluted in different solvents such as chloroform, methanol, ethyl acetate and grain alcohol. In order to determine the antimicrobial potential of extracts, agar well diffusion method was used, with controls for each diluent. After that, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods were used. All tests were performed in triplicate. In the agar well diffusion test, the measurements of the inhibition zone for propolis extract were as follows: grain alcohol and propolis (2.88mm), methanol and propolis (2.41mm), chloroform and propolis (2.40mm) and ethyl acetate and propolis (0.83mm). The MBC of propolis extracts in different solvents were 93.75 μg/mL for grain alcohol, 375 μg/mL for chloroform and methanol and 3,000 μg/ml for ethyl acetate. Statistically significant differences were achieved comparing the inhibition zones of propolis diluted in grain alcohol and ethyl acetate (2.88 and 0.83 mm, respectively). Considering the low cost of therapy and the activity of the propolis against caprine mastitis pathogens, other studies regarding in vivo activity and chemical characterization are necessary, in addition to evaluation of the toxicological aspects of propolis extracts.The purpose of the present study was to evaluate the antimicrobial activity of propolis extracts diluted in different solvents against bacteria from Staphylococcus genus. The study was performed in the Immunology and Microbiology Laboratory from Universidade Federal do Vale do S o Francisco. The propolis extracts were prepared using brown propolis diluted in different solvents such as chloroform, methanol, ethyl acetate and grain alcohol. In order to determine the antimicrobial potential of extracts, agar well diffusion method was used, with controls for each diluent. After that, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods were used. All tests were performed in triplicate. In the agar well diffusion test, the measurements of the inhibition zone for propolis extract were as follows: grain alcohol and propolis (2.88mm), methanol and propolis (2.41mm), chloroform and propolis (2.40mm) and ethyl acetate and propolis (0.83mm). The MBC of propolis extracts in
Ocorrência e fatores de risco para Chlamydophilaabortus em ovinos e caprinos no estado de Pernambuco
Pereira, Márcia de Figueiredo;Peixoto, Rodolfo de Moraes;Piatti, Rosa Maria;Medeiros, Elizabeth Sampaio de;Mota, Iagmar Oliveira da;Azevedo, Sérgio Santos de;Mota, Rinaldo Aparecido;
Pesquisa Veterinária Brasileira , 2009, DOI: 10.1590/S0100-736X2009000100005
Abstract: the study aimed to identify risk factors associated with chlamydophila abortus infection in sheep and goats from the litoral/zona da mata and agreste region of pernambuco state, brazil. serum samples (n=290) were analyzed to detect chlamydophila spp. antibodies in 12 farms. questionnaires were applied to identify risk factors. frequency of serum-reactive animals were 10.3% (12.0% in ewes and 8.1% in goats) and 1/12 (91.6%) infection focuses were identified. this is the first report on anti-chlamydophhila abortus antibodies in goats and sheep in pernambuco and brazil, respectively. risk factors associated with goat infection were breed (or=9.10) and management (or=6.41). no significant associations in any of the analyzed factors were found for sheep. in summary, chlamydophila sp. infection is disseminated in sheep and goat herds in the region. control measures should be established, focusing primarily risk factor identified in this study, to reduce the possibility of infection by the agent.
Sensibilidade antimicrobiana de bactérias isoladas de Jundiá (Rhamdia quelen)
Costa, Mateus Matiuzzi da;Peixoto, Rodolfo de Moraes;Boijink, Cheila de Lima;Castagna, Lucélia;Meurer, Fabio;Vargas, Agueda Castagna de;
Pesquisa Veterinária Brasileira , 2008, DOI: 10.1590/S0100-736X2008001000006
Abstract: aiming the evaluation of sensitivity profiles of pathogen bacteria responsible for fish diseases, 51 bacterial isolates from jundiá (rhamdia quelen) belonging to the genus acinetobacter spp. (8), aeromonas spp. (15), edwardsiella spp. (2), enterobacter spp. (2), klebsiella spp. (1), plesiomonas spp. (5), pseudomonas spp. (1), staphylococcus spp. (11), and vibrio spp. (6), were tested against antimicrobial agents used for treatment of bacterial fish diseases. all samples were processed at the laboratory of bacteriology, department of preventive veterinary medicine, ufsm. from 51 bacteria isolated from jundiá fishes (rhamdia quelen) 51 (100%) were sensitive to gentamycin, 49 (96,08%) to sulphazotrin, 47(92,16%) to chloramphenicol, 43 (84,31%) to tetracylin, 43 (84,31%) to naldixic acid, 31 (60,78%) to nitrofurantoin, 22 (43,14%) to erytromycin, 22 (43,14%) to ampicillin, 15 (29,41%) spiramycin, 13 (25,50%) to cholystin, and 5 (3%) to penicillin g. with exception of an isolate of staphylococcus spp., the bacteria analyzed in the present study were resistant to one or more antimicrobial agents tested. knowledge of the sensitivity profile of bacteria involved in infectious processes in fish will allow rational antimicrobial treatment that will contribute to the control of fish diseases in rhamdia quelen without causing great risks to public health and the environment.
Dermatophytosis caused by Microsporum canis and Microsporum gypseum in free-living Bradypus variegatus (Schiz, 1825) in the state of Pernambuco, Brazil
Xavier, Gileno Ant？nio Araújo;Silva, Leonildo Bento Galiza da;Silva, Davi Rubem da;Peixoto, Rodolfo de Moraes;Lino, Gileno Camara;Mota, Rinaldo Aparecido;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000300018
Abstract: three cases of dermatophytosis in free living brown-throated three-toed sloths (bradypus variegatus) in the zona da mata, north of pernambuco state, brazil, were studied. two animals presented areas of alopecia on the pelvic member and thorax and one animal on the pelvic member only. the three animals presented scabs. hair and scabs samples were submitted to microscopical examination after treatment with a 30 % koh and cultivated in mycosel agar. the direct examination indicated the presence of arthrospores in the hair. colonies grown after seven days of culture were confirmed as microsporum based on examination of the structure of the macroconidia. this is the first observation of dermatophytosis caused by microsporum canis and microsporum gypseum in free living sloths in the state of pernambuco.
Fatores de risco associados à infec？？o por Toxoplasma gondii em ovinos e caprinos no estado de Pernambuco
Pereira, Márcia de Figueiredo;Peixoto, Rodolfo de Moraes;Langoni, Hélio;Greca Junior, Haroldo;Azevedo, Sérgio Santos de;Porto, Wagnner José Nascimento;Medeiros, Elizabeth Sampaio de;Mota, Rinaldo Aparecido;
Pesquisa Veterinária Brasileira , 2012, DOI: 10.1590/S0100-736X2012000200009
Abstract: this study was conducted to investigate the participation of toxoplasma gondii in reproductive failure in small ruminants raised in the litoral/ zona da mata and agreste of the state of pernambuco, brazil. twelve flocks were selected from which 262 samples were collected, 167 from goats and 95 from sheep. indirect imunofluorescent antibody reaction (ifa) technique was used to detect antibodies anti-toxoplasma gondii. a questionnaire was applied in flocks to identify risk factors associated with infection. positive animals were found in 100% of the farms. among 167 samples of goat serum analyzed, 31.7% were positive, whereas from 95 samples of sheep, 16.9% were positive. results of brucellosis tests were negative for 100% of analyzed samples. for sheep, no significant association was found; however, for goats, there was a significant association (p<0.05) for intensive handling (or=2.40), milk exploration (or=2.10), animals originating from other states (or=7.89) and natural breeding (or=5.69). it was concluded that infection for t. gondii is spread in studied goat and sheep flocks and that sanitary measures must be adopted to control risk factors for infection.
Effects of Post-Training Blockade of GABAB Receptor on Memory of Food Location and Expression of Synapsin I in the Hippocampus of Pigeons (Columba livia)  [PDF]
Fernando Canova, Rodolfo Souza Faria, Elenice Aparecida de Moraes Ferrari
Journal of Behavioral and Brain Science (JBBS) , 2014, DOI: 10.4236/jbbs.2014.412055
Abstract: This study investigated effects of post-training treatment with phaclofen, GABAB receptor antagonist, on the memory of food location and on the expression of Synapsin I in the hippocampus of pigeons. Pigeons were trained in food location (7 sessions) and underwent post-training treatment with phaclofen (0.3 mg/kg, i.p.; PHAC), saline (SAL) or non-treated (NTR). Testing for memory persistence occurred 7 days after the last training session (PHACR, SALR and NTRR Groups). Pigeons treated with phaclofen had lower latency and higher correct choice values than saline and non-treated controls (p < 0.05). Analysis of hippocampus tissue indicated that Synapsin I-positive cell counts were higher in pigeons treated with phaclofen than in saline and non-treated controls (p < 0.05). Data indicated enhancement of consolidation and persistence of food location memory, and up-regulation of Synapsin I expression in the hippocampus of pigeons, which were related with post-training blockade of GABAB receptors.
BIOCHEMICAL CHARACTERIZATION AND ANTIBIOTICS SENSIBILITY OF BACTERIA AGENTS RESPONSIBLE FOR OVINE SUBCLINICAL MASTITIS IN WEST REGION OF THE SANTA CATARINA CARACTERIZA O BIOQUíMICA E PERFIL DE SENSIBILIDADE AOS ANTIMICROBIANOS DE AGENTES BACTERIANOS ISOLADOS DE MASTITE SUBCLíNICA OVINA NA REGI O OESTE DE SANTA CATARINA
Guilherme Drescher,Samara Paula Mattiello,Rodolfo Moraes Peixoto,Agueda Castagna Vargas
Ciência Animal Brasileira , 2010,
Abstract: Ovine mastitis is an infectious disease associated to economic losses in dairy and meat sheep. The present study aims to determine the antimicrobial drug patterns of the most frequent etiologic agents of ovine mastitis. We analyzed 163 milk samples using Whiteside test, besides the bacteriological culture in ovine blood agar 5% (AS). From 163 analyzed samples 23.31% and 14.72% were positive and strongly positive, according to the Whiteside test. The most frequent etiologic agents of ovine mastitis isolated in the present study were: Staphylococcus spp. (31.39%), Acinetobacter spp. (10.50%), Enterobacter aerogenes (10.50%), Escherichia coli (8.20%) and Aeromonas spp. (6.70%). The sensitivity patterns to antimicrobial drugs showed high variation. The small sensitivity was observed to novobiocin (10.46%). The higher sensitivity was detected to lyncomicin + streptomicin association (86.02%). In our study we conclude that Staphylococcus spp. are the most frequent bacterial agents envolved in subclinical mastitis in sheep. The association of lyncomicin + streptomicin and josamicin + thrimetoprin were more efficient in vitro sensitivity tests. KEY WORDS: Mastitis, microrganisms, antimicrobial resistance, Whiteside test. A mastite é uma enfermidade que acomete principalmente animais com aptid o leiteira, ocasionando uma série de prejuízos aos produtores de ovinos de leite e corte. Objetivou-se avaliar o perfil de sensibilidade aos antimicrobianos dos principais agentes causadores de mastite em ovelhas. Foram analisadas 163 amostras de leite, sendo utilizado o teste Whiteside Modificado (WSM), além do cultivo das amostras em ágar sangue ovino 5% (AS). Das 163 amostras analisadas, observou-se que 23,31% e 14,72% foram positivas e fortemente positivas, respectivamente, no teste WSM. Os principais agentes isolados foram Staphylococcus spp. (31,39%), Acinetobacter spp. (10,50%), Enterobacter aerogenes (10,50%), Escherichia coli (8,20%) e Aeromonas spp. (6,70%). O perfil de sensibilidade aos antimicrobianos apresentou grande varia o, com a novobiocina demonstrando o menor percentual de sensibilidade (10,46%). A maior taxa de susceptibilidade foi observada para a associa o de lincomicina com estreptomicina (86,02%). Neste estudo, observou-se que os Staphylococcus spp. s o os agentes comumente envolvidos nos casos de mastite subclínica em ovelhas. A associa o entre lincomicina e estreptomicina e josamicina e trimetoprim constituiu as drogas mais eficientes nos testes de sensibilidade in vitro contra os agentes da mastite ovina. PALAVRAS-CHAVES: Mastite, microrgani
Invisibilidade, preconceito e violência racial em Curitiba
Moraes, Pedro Rodolfo Bodê de;Souza, Marcilene Garcia de;
Revista de Sociologia e Política , 1999, DOI: 10.1590/S0104-44781999000200001
Abstract: o artigo prop？e-se analisar o processo de constru？？o de curitiba enquanto "capital européia" o que, por sua vez, implicou tornar invisível a popula？？o negra. demonstra-se como esta imagem, que pode ser vislumbrada através do projeto arquitet？nico da cidade, tem suas origens em um falsa idéia de que o paraná constituiu-se como um "brasil diferente", que n？o teria tido a mesma configura？？o racial que o restante do país. principalmente porque, segundo o argumento de intelectuais e pesquisadores locais, n？o houve escravid？o nem popula？？o negra significativa. argumentamos que estas conclus？es s？o incorretas e que tanto no passado quanto hoje há um contigente populacional de negros que s？o tornados invisíveis, com consequências negativas para este grupo, na assim chamada "capital das etnias".
Estratégia competitiva para empresas produtoras de hortículas no sistema organico The successful strategy of a grower and distributor of vegetables
Cleber Luiz Ferreira Dantas,Rodolfo Araújo de Moraes Filho
Rege : Revista de Gest？o , 2006,
Abstract: O presente trabalho apresenta os resultados de uma análise da estratégia adotada por uma empresa produtora e distribuidora de hortali as organicas e tradicionais que atua no mercado da regi o metropolitana do Grande Recife, Estado de Pernambuco. Essa empresa optou pela estratégia competitiva genérica de diferencia o, enfocando a produ o e a comercializa o com mais eficiência que as demais organiza es do setor. O foco do estudo é a estratégia tática da empresa, que enfatizou a tecnologia de produ o e de distribui o. Nele, consegue-se identificar os tipos de interven o técnica e também os aspectos administrativos de agrega o de valor aos produtos que conferem vantagens comerciais ao produtor, com o fornecimento de hortali as de melhor qualidade, demandadas por um cliente-tipo de maior poder aquisitivo. Neste artigo, analisa-se o sucesso da estratégia de diferencia o como fator de competitividade para o ramo hortícola, tendo como estudo de caso o Sítio Vertentes, produtor de hortali as organicas, convencionais e hidrop nicas, localizado no município de Ch Grande, agreste Pernambucano. Os fundamentos teóricos deste estudo s o os conceitos básicos da estratégia competitiva. Differentiation, as a successful competitive strategy, was analyzed in the "Sitio Vertentes" which grows conventional, hydroponic and organic vegetables in rural Pernambuco to distribute them in Metropolitan Recife. The focus was on better production, distribution and commercialization efficiency where technical and administrative actions added value and commercial advantages in supplying the higher quality vegetables demanded by customers with enhanced purchasing power. The technical fundamentals of this study are the basic concepts of competitive strategy.
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